Rottapharm Biotech, a research company dedicated to the discovery and development of innovative drugs in different therapeutic areas, such as rheumatology (osteoarthritis and rheumatoid arthritis), pain and inflammation, immunology and immuno-oncology, is seeking a researcher to join the R&D; team. The researcher will be involved in the discovery and development of next-generation AAV capsids for use in gene therapies targeting osteoarthritis (OA) tissues.
**Key Responsibilities**: - Prepares and maintains cell cultures (including primary cell cultures), perform studies and experiments including tissue processing, biochemical analyses, DNA, RNA and protein isolation, qRT-PCR, ELISA, immunoblot processing.
- Plan and execute in vitro and vivo screening campaigns in human OA tissues and animal OA models; analyze biodistribution and transduction profiles across tissues.
- Develop and implement in vitro screening assays, including binding assays and transduction readouts in relevant cell lines.




- Collaborate with cross-functional teams on vector design, production, and payload optimization.
- Data analysis and management.
- Maintain accurate records of experimental design and outcomes; contribute to internal reports, presentations, and publications. **Qualifications**:
Strong molecular biology and cell culture skills. Skills include mammalian cell culture (stable and adherent lines), vector engineering, genetic engineering, PCR, transfection, transduction, and assay development.
- Strong foundation in AAV biology and capsid structure-function.
- Experience with high-throughput screening, and NGS-based analysis is preferred.
- Prior work with in vivo models is preferred.
- Experience in FACS analysis and IHC is a plus;
- Analysis, accuracy, confidential skills and a strong attitude to reach the company’s achievements.

📌 Ricercatore Biologia Molecolare e Cellulare (Monza)
🏢 ROTTAPHARM BIOTECH
📍 Monza