The research group of Precision Medicine led by Prof. Salvatore
Piscuoglio (
[email protected] [
[email protected]])
will be based al the Humanitas University, Milan, Italy, from July 2024 is
seeking one highly motivated Laboratory Technicians in Cell Biology (100%) to
join our team of bioinformaticians, molecular biologists, pathologists, and
clinical fellows.
The candidates will develop, optimize and apply cutting-edge cellular/ molecular
methods to generate 3D in vitro and ex vivo tumor models that adequately
recapitulate the tumor architecture and mimic the cell-to-cell interactions
within the tissue microenvironment. The specific tasks will include the
generation and maintenance of patient-derived tumor organoids, patient-derived
tumor fragments, fibroblasts, endothelial cells, Peripheral Blood Mononuclear
Cells (PBMCs), and Tumor Infiltrating Lymphocytes (TILs).
Main responsibilities include
* processing patient-derived samples according to pre-established guidelines
(e.g., tissue dissociation, isolation of PBMCs/ TILs, endothelial cells,
sample freezing and storage, nucleic acids and protein extraction, and
patient-derived tumor organoid/ fragment generation and maintenance)
* generation and maintenance of cell cultures and execution of experiments on
cell cultures
* updating the database of patient samples and reagents
* documenting the experiments
* providing general support for lab maintenance within the scope of this
project
Profile
* "Laboratory Technician" degree or a Bachelor/Master degree in molecular
biology, biotechnology or a related discipline
* excellent technical expertise in molecular/ cell biology techniques
* hands-on experience handling and processing patient samples
* hands-on experience with cell culture and flow cytometry techniques is a must
* experience in primary cell culture and 3D cultures is highly desirable
* technical expertise in organoid generation and maintenance is a plus
* once trained, the abil
📌 Laboratory Technician Cell Biology (Rozzano)
🏢 Humanitas
📍 Rozzano