A Research Fellow position is immediately available under the supervision of Michela Milani, Project Leader in the group led by Alessio Cantore at the San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), Milan, Italy ()
The Institute.
SR-Tiget was created in **** as a joint venture between Fondazione Telethon and Ospedale San Raffaele (OSR), with the mission to perform cutting-edge
research on gene and cell therapy and to translate its results into therapeutic advances for genetic diseases
. The Institute is located within the OSR campus, which includes a large multi-disciplinary research hospital, a biomedical research center, a university, and also hosts several biotechnology companies. Over the years, SR-Tiget has given a
pioneering contribution to the gene and cell therapy field
with relevant discoveries in vector design, gene transfer and gene editing strategies, stem cell biology, identity and mechanism of action of regulatory cells in immune responses, and innate immune cells in cancer. SR-Tiget has also established the resources and framework for
translating these advances into novel experimental therapies
and has implemented several successful gene therapy clinical trials, which have already treated >140 patients and led to the filing and approval of 2 novel advanced therapy medicines.
Posizione:
The position.
We are looking for a motivated
Research Fellow
to work on an exciting new project aiming at developing
an innovative
in vivo
lentiviral vector gene therapy strategy for hemophilia A
. In particular, we will develop different lentiviral vectors encoding an engineered version of human factor VIII (FVIII) transgene under the control of different megakaryocyte-specific promoters. These vectors will be tested
in vitro
and
in vivo
in hemophilia A mouse models to select the best candidate to achieve
effective FVIII expression in platelets after
in vivo




gene therapy to hematopoietic stem and progenitor cells
. This project stems from our solid and extensive experience in
in vivo
gene therapy for hemophilia and our recent discovery of a window of opportunity in newborns to target
in vivo
hematopoietic stem and progenitor cells by intravenously administered lentiviral vectors.
Main tasks
- Molecular cloning of new LV constructs targeting FVIII expression to megakaryocytes
- Production and
in vitro
characterization of recombinant lentiviral vectors
-
In vivo
testing in mouse models of hemophilia A of the generated LV
- Immunological assays to evaluate immunogenicity of the developed strategy
- Data analysis and critical interpretation of the results
- Discussion of project results at internal and external meetings
Requisiti:
Qualifications and skills
- Degree in biology/biotechnology (top grades) and research experience in competitive laboratories
- The selected candidate is expected to apply for a Ph.D. program in the future
- Cell culture and molecular biology experience
- Previous experience working with mice is considered a plus
- High accuracy, reliability, precision
- High motivation to work in an international environment
- Excellent organizational skills
- Friendly, collaborative, pro-active mindset
- Good oral and written English skills
Altre informazioni:
We offer
- 1-year renewable fixed-term contract (assunzione a tempo determinato)
- RAL ****** €
- Opportunity to learn new cutting-edge advanced techniques
- A dynamic environment and the chance to give an essential contribution to the clinical translation of innovative ATMP
Applications, including a CV and the names and contact details of at least one referee, should be sent by email to
******.
Evaluation of applications/interviews will begin immediately and continue until the position is filled. The selected candidate is expected to start their role in January ****.

📌 Research Fellow (Milano)
🏢 Fondazione Telethon ETS
📍 Milano